Detection of Disease Resistance Genes in 233 Medium Fruit Tomato Isolates

doi:10.11924/j.issn.1000-6850.casb2024-0012

Abstract

Abstract:

In order to screen the resources of medium fruit tomato with multiple resistances and provide material basis for tomato disease resistance breeding, using 28 medium fruit tomato varieties introduced by our research group as materials, 17 resistance genes of 12 diseases were detected by five-primer amplification inhibited mutation system (PARMS) method, including yellow leaf curl virus resistance genes Ty-1 and Ty-5, tobacco mosaic virus resistance gene Tm-2^a, root nematode resistance genes Mi1.2 and Mi2.3, Fusarium crown and root rot resistance gene Frl, leaf mold resistance genes Cf-5 and Cf-9, grey leaf spot resistance genes Sm, wilt resistance gene I-2, late blight resistance gene Ph-3, bacterial wilt resistance genes Bwr12 and Bwr6, bacterial speck resistance gene Pto, bacterial spot resistance gene Rx4, Verticillium wilt resistance gene Ve1 and Ve2. Among the 233 isolates, there were 222, 215, 198 and 194 isolates containing the resistance genes Ve1, Sm, Ve2 and Mi1.2, respectively. The isolates with 7 and 8 resistance genes were 55 and 50, respectively. 140 isolates were with 8 or more resistance genes, accounting for 60%. 3 isolated strains containing 12 resistance genes were screened, which were 2-2, 5-4 and 21-3. And there were 21 isolated strains containing 11 resistance genes, which were 1-1, 1-3, 2-3, 2-4, 2-7, 2-8, 4-2, 4-4, 4-5, 5-7, 5-8, 8-1, 8-7, 8-8, 12-1, 12-2, 12-5, 18-7, 18-8, 20-8, 26-2. Polyresistant tomato resources were abundant. In this study, a number of medium fruit tomato resources with multiple antibodies were obtained, and the next step can be purification of the isolated single strain, providing a material basis for screening medium fruit tomato with multiple antibodies.

Key words: medium-fruit tomato, isolates, PARMS, resistance detection

WANG Shanshan, WANG Chenyu, YANG Chaosha, CAI Xiaoyi, YIN Qingzhen. Detection of Disease Resistance Genes in 233 Medium Fruit Tomato Isolates[J]. Chinese Agricultural Science Bulletin, 2024, 40(34): 24-32.

Figures/Tables 15

References 28

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编号	品种名称	编号	品种名称
1	2122 F₂-1-3	15	303 F₂-3-1
2	JY 213 F₂-5-1	16	CX F₂-1-5
3	JM 1号F₂-2-6	17	ML F₂-2-3
4	JM 2号F₂-1-5	18	CM 1号F₂-8-2
5	FQ 4号F₂-1-8	19	ZHLB F₂-9-2
6	T2204 F₂-3-3	20	YMGT F₂ -7-4
7	口感9号F₂-5-3	21	36009 F₂-5-8
8	HL 15号F₂-4-1	22	39717 F₂-4-4
9	HL 16号F₂-7-1	23	FBY 1317 F₂-6-1
10	HX F₂-4-8	24	TJ 1号F₂-4-2
11	166-2号F₂-1-2	25	TJ 2号F₂-1-8
12	CM三号F₂-6-2	26	TJ 3号F₂-2-4
13	2138 F₂-2-3	27	TJ 4号F₂-3-1
14	NG 803 F₂-4-5	28	TJ 5号F₂-4-2

编号	品种名称	编号	品种名称
1	2122 F₂-1-3	15	303 F₂-3-1
2	JY 213 F₂-5-1	16	CX F₂-1-5
3	JM 1号F₂-2-6	17	ML F₂-2-3
4	JM 2号F₂-1-5	18	CM 1号F₂-8-2
5	FQ 4号F₂-1-8	19	ZHLB F₂-9-2
6	T2204 F₂-3-3	20	YMGT F₂ -7-4
7	口感9号F₂-5-3	21	36009 F₂-5-8
8	HL 15号F₂-4-1	22	39717 F₂-4-4
9	HL 16号F₂-7-1	23	FBY 1317 F₂-6-1
10	HX F₂-4-8	24	TJ 1号F₂-4-2
11	166-2号F₂-1-2	25	TJ 2号F₂-1-8
12	CM三号F₂-6-2	26	TJ 3号F₂-2-4
13	2138 F₂-2-3	27	TJ 4号F₂-3-1
14	NG 803 F₂-4-5	28	TJ 5号F₂-4-2

抗性基因个数	资源份数	占比/%
3	3	1.3
4	2	0.9
5	10	4.3
6	22	9.4
7	55	23.6
8	50	21.5
9	39	16.7
10	28	12.0
11	21	9.0
12	3	1.3

抗性基因个数	资源份数	占比/%
3	3	1.3
4	2	0.9
5	10	4.3
6	22	9.4
7	55	23.6
8	50	21.5
9	39	16.7
10	28	12.0
11	21	9.0
12	3	1.3