Effect of Density on Survival of Hippodamia variegata Larva

doi:10.11924/j.issn.1000-6850.casb2024-0070

Abstract

Abstract:

In order to clarify the effects of feeding density on the development period, pupa rate, adult rate and weight of Hippodamia variegata, as well as to explore efficient feeding techniques for this species, four groups of early-stage larvae were subjected to different feeding densities (A: 100 heads/box, B: 200 heads/box, C: 300 heads/box, D: 400 heads/box, the volume of the breeding box is 1000 mL). The molting, pupa, eclosion time and the number of pupation and eclosion adults of each age of H. variegata were observed and counted. The results showed that the development duration of larvae and pupa of H. variegata in groups A, B, C, and D were (11.75±0.65) days, (12.06±0.77) days, (12.45±0.60) days, and (12.70±0.21) days, respectively. The number of pupation was (85.25±4.87), (147.50±3.12), (193.75±21.40) and (156.25±29.62), of which (49.00±6.68), (74.25±12.06), (107.75±16.76) and (103.75±15.78) pupae could be collected. The number of eclosion adults was (75.25±6.73) heads, (123.75±8.05) heads, (160.25±27.22) heads and (110.75±26.68) heads, respectively. The weight of adults was (7.39±0.13) mg/head, (7.32±0.29) mg/head, (7.29±0.20) mg/head and (6.56±0.12) mg/head, respectively, and the number of abnormal adults was (5.25±1.65) heads, (12.75±4.33) heads, (19.50±5.24) heads, (10.50±3.90) heads, respectively. With the increase of feeding density, the development period of H. variegata was gradually prolonged, the number of pupation, eclosion and deformity was gradually increased, and the pupation rate, eclosion rate and adult weight gradually decreased. In conclusion, the instar larvae of H. variegata had a feeding density of 300 heads/box, and the number of pupation and eclosion adults was large, and the weight was normal, which was the optimal feeding density.

Key words: aphids, Hippodamia variegata, stocking density, biological control

YONG Yanping, XU Bingyan, LIU Hongling, LI Yang, HE Hengguo, PU Deqiang. Effect of Density on Survival of Hippodamia variegata Larva[J]. Chinese Agricultural Science Bulletin, 2024, 40(27): 115-120.

Figures/Tables 5

References 26

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处理	幼虫期				蛹前期	蛹期	总历期
处理	1龄	2龄	3龄	4龄	蛹前期	蛹期	总历期
A	1.13±0.25 b (1.0~1.5)	2.00±0.41 a (1.5~2.5)	1.63±0.25 b (1.5~2.0)	2.63±0.48 a (2.0~3.0)	0.88±0.25 a (0.5~1.0)	3.50±0.41 a (3.0~4.0)	11.75±0.65b (11.0~12.5)
B	1.06±0.13 b (0.88~1.13)	1.50±0.41 ab (1.0~2.0)	2.00±0.58 ab (1.5~2.5)	3.00±0.41 a (2.5~3.5)	1.25±0.29 a (1.0~1.5)	3.25±0.65 a (2.5~4.0)	12.06±0.77ab (11.38~13.13)
C	1.42±0.03 a (1.38~1.44)	1.25±0.50 b (1.0~2.0)	2.13±0.48 ab (1.5~2.5)	3.25±0.29 a (3.0~3.5)	1.13±0.25 a (1.0~1.5)	3.28±0.66 a (2.5~4.0)	12.45±0.60ab (11.94~13.0)
D	1.16±0.24ab (1.0~1.5)	1.38±0.25b (1.0~1.5)	2.63±0.25a (2.5~3.0)	3.25±0.50a (3.0~4.0)	1.38±0.48a (1.00~1.50)	2.92±0.73a (2.00~3.67)	12.70±0.21a (12.50~13.0)

处理	幼虫期				蛹前期	蛹期	总历期
处理	1龄	2龄	3龄	4龄	蛹前期	蛹期	总历期
A	1.13±0.25 b (1.0~1.5)	2.00±0.41 a (1.5~2.5)	1.63±0.25 b (1.5~2.0)	2.63±0.48 a (2.0~3.0)	0.88±0.25 a (0.5~1.0)	3.50±0.41 a (3.0~4.0)	11.75±0.65b (11.0~12.5)
B	1.06±0.13 b (0.88~1.13)	1.50±0.41 ab (1.0~2.0)	2.00±0.58 ab (1.5~2.5)	3.00±0.41 a (2.5~3.5)	1.25±0.29 a (1.0~1.5)	3.25±0.65 a (2.5~4.0)	12.06±0.77ab (11.38~13.13)
C	1.42±0.03 a (1.38~1.44)	1.25±0.50 b (1.0~2.0)	2.13±0.48 ab (1.5~2.5)	3.25±0.29 a (3.0~3.5)	1.13±0.25 a (1.0~1.5)	3.28±0.66 a (2.5~4.0)	12.45±0.60ab (11.94~13.0)
D	1.16±0.24ab (1.0~1.5)	1.38±0.25b (1.0~1.5)	2.63±0.25a (2.5~3.0)	3.25±0.50a (3.0~4.0)	1.38±0.48a (1.00~1.50)	2.92±0.73a (2.00~3.67)	12.70±0.21a (12.50~13.0)