Effect of Bacillus on Growth Performance, Digestion, Non-Specific Immunity and Disease Resistance of Procambarus clarkii

doi:10.11924/j.issn.1000-6850.casb2024-0186

Abstract

Abstract:

To study the effects of Bacillus on the growth performance, digestion, non-specific immunity and disease resistance of Procambarus clarkii, a total of 270 P. clarkii with body weight of (9.36±1.40) g were selected and divided into 3 groups of CK (without Bacillus) group, T₁ (with B. coagulans) group and T₂ (with B. subtilis) group, for a 4-week culture experiment. The results showed that there were no significant differences in final body weight among all groups (P>0.05), while WGR, SGR, SR and HSI in groups T₁ and T₂ were significantly higher than those in group CK (P<0.05). The activities of ACP, ALP, LZM and SOD in groups T₁ and T₂ were significantly higher than those in group CK (P<0.05). The LPS and TPS activities of groups T₁ and T₂ were significantly higher than those of group CK (P<0.05), and the AMS activity of group T₁ was significantly higher than that of groups CK and T₂ (P<0.05), but there was no significant difference between groups T₂ and CK (P>0.05). After WSSV challenge, the survival rate of P. clarkii in groups T₁ and T₂ was significantly higher than that in group CK (P<0.05). In conclusion, the two species of bacilluscan significantly improve the growth performance of P. clarkii, increase intestinal digestive enzyme and serum non-specific immunoenzyme activity, enhance disease resistance and reduce mortality when infected with WSSV.

Key words: Procambarus clarkii, Bacillus coagulans, Bacillus subtilis, non-specific immunity, disease resistance

SHAN Jinfeng, WU Chun, WANG Teng, WANG Zeping, WANG Xinhai. Effect of Bacillus on Growth Performance, Digestion, Non-Specific Immunity and Disease Resistance of Procambarus clarkii[J]. Chinese Agricultural Science Bulletin, 2024, 40(31): 159-164.

Figures/Tables 4

References 46

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组别	初始体重/g	终末体重/g	WGR/%	SGR/(%/d)	SR/%	HSI/%
CK	9.36±1.40	15.66±0.26	67.30±1.57b	1.37±0.08b	86.67±2.63b	5.42±0.23c
T₁	9.36±1.40	16.27±0.18	73.82±1.65a	1.49±0.03a	92.23±2.89a	7.73±0.57b
T₂	9.36±1.40	16.85±0.23	80.02±1.71a	1.52±0.04a	91.11±2.74a	8.26±0.35a

组别	初始体重/g	终末体重/g	WGR/%	SGR/(%/d)	SR/%	HSI/%
CK	9.36±1.40	15.66±0.26	67.30±1.57b	1.37±0.08b	86.67±2.63b	5.42±0.23c
T₁	9.36±1.40	16.27±0.18	73.82±1.65a	1.49±0.03a	92.23±2.89a	7.73±0.57b
T₂	9.36±1.40	16.85±0.23	80.02±1.71a	1.52±0.04a	91.11±2.74a	8.26±0.35a

组别	ACP	ALP	LZM	SOD
CK	75.25±4.06b	52.04±6.73b	30.26±0.71c	154.33±3.82c
T₁	93.47±5.12a	66.94±7.43a	42.85±0.93a	196.37±2.48b
T₂	91.06±3.47a	69.75±5.21a	38.22±1.37b	232.72±4.41a

组别	ACP	ALP	LZM	SOD
CK	75.25±4.06b	52.04±6.73b	30.26±0.71c	154.33±3.82c
T₁	93.47±5.12a	66.94±7.43a	42.85±0.93a	196.37±2.48b
T₂	91.06±3.47a	69.75±5.21a	38.22±1.37b	232.72±4.41a

组别	AMS	LPS	TPS
CK	0.82±0.11b	98.04±0.02b	4.18±0.39b
T₁	0.91±0.18a	216.45±3.09a	5.64±0.87a
T₂	0.84±0.24b	236.33±4.14a	5.83±1.09a