Abstract:

In order to definite the species of virus, which cause tomato leaf samples chlorosis necrosis, fruit necrosis, and concentric spots, we collected the susceptible tomato samples, and pepper leaf samples exhibiting symptoms of brown wilting and necrosis, fruit deformity and shriveling. RT-PCR detection was performed utilizing specific primers targeting the tomato spotted wilt virus (TSWV) as well as N gene primers. The results indicated that occurrence of TSWV infection in both tomato and pepper samples. Phylogenetic analysis based on the N gene sequence demonstrated a close relationship between TSWV isolates obtained from tomatoes and peppers in Shaanxi with those found in Australian peppers, Japanese tomatoes, as well as Beijing Verbenas within China. During the virus inoculation experiment, tomato and pepper samples both displayed characteristic symptoms including chlorosis, necrotic foliage, and shriveled fruits; RT-PCR successfully detected the presence of TSWV in these plants. This study confirms that tomatoes and peppers in Shaanxi have been affected by TSWV infection and provides a theoretical basis for its prevention and control.

Key words: Shaanxi Province, tomato, pepper, tomato spotted wilt virus, molecular identification