Effect of Temperature on Infection Process of Pseudoperonospora cubensis in Cucumber

doi:10.11924/j.issn.1000-6850.casb2024-0528

Abstract

Abstract:

The effects of different temperatures on the release of sporangia and spore germination of the pathogen of Pseudoperonospora cubensis were discussed. The infection process of P. cubensis in leaves was observed by Trypan blue staining and scanning electron microscopy, which laid a theoretical foundation for early monitoring and early warning of cucumber downy mildew. The study found that the release rates of sporangia of P. cubensis at 5, 10℃ were (81.57±1.91)% and (71.06±1.65)%, respectively, significantly higher than other temperature conditions. At 15, 20℃, the spore germination rates of P. cubensis were significantly higher than that at other temperatures, with (51.91±1.97)% and (46.01±2.02)%, respectively. After inoculation with P. cubensis, the zoospores adhered to stomata and began to invade the host within 0-4 hours. From 4-6 hours, hyphae formed haustoria in the intercellular spaces of mesophyll cells to absorb tissue nutrients. At 12-24 hours, the hyphae developed into conidiophores, and after 24 hours, sporangia were produced, releasing zoospores for reinfection. This study clarifies the optimal temperature range for the release of sporangia and spore germination of P. cubensis, as well as the structural characteristics of each stage of P. cubensis infection. It provides theoretical support for the early monitoring and warning of cucumber downy mildew and the research of ecological prevention and control technologies.

Key words: Pseudoperonospora cubensis, cucumber downy mildew, sporangium, spore germination, temperature, infection process, monitoring and early warning

LI Yaqi, LIU Huiqin, YANG Lijuan, WANG Yong, GAO Wei. Effect of Temperature on Infection Process of Pseudoperonospora cubensis in Cucumber[J]. Chinese Agricultural Science Bulletin, 2025, 41(13): 147-151.

Figures/Tables 4

References 25

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