Arachis hypogaea L. Embryo Leaflet: Establishment and Optimization of in Vitro Regeneration System

doi:10.11924/j.issn.1000-6850.casb2025-0050

Abstract

Abstract:

The purpose of this study was to establish an efficient in vitro regeneration system of embryo lobules of different peanut varieties, and to provide strong technical support for genetic improvement and biotechnology application of peanut. In this experiment, the embryo lobules of two different peanut varieties ‘Laiyang Silihong’ and ‘83’ (‘Yunhua 24’) were used as explants, and the effects of different plant growth regulators and seedling hardening time on the establishment of embryo lobule regeneration system were studied by tissue culture method. The results showed that the most suitable growth regulator for ‘Laiyang Silihong’ was 3.25 mg/L 6-BA+0.2 mg/L NAA for bud cluster induction, 1.5 mg/L TDZ+3.0 mg/L 6-BA+1.0 mg/L GA₃ for bud cluster extraction, 2.0 mg/L TDZ+4.0 mg/L 6-BA+0.1 mg/L NAA+1.5 mg/L GA₃ for bud cluster elongation, and 2.0 mg/L NAA+0.3 mg/L 2,4-D for rooting culture. The most suitable growth regulator for ‘83’ (‘Yunhua 24’) embryo leaflet bud induction was 3.25 mg/L 6-BA+0.3 mg/L NAA, the bud cluster was 1.5 mg/L TDZ+4.0 mg/L 6-BA+1.5 mg/L GA₃, the bud cluster elongation was 2.0 mg/L TDZ+3.5 mg/L 6-BA+1.0 mg/L NAA+2.0 mg/L GA₃, and the rooting culture was 2.5 mg/L NAA+0.1 mg/L 2,4-D. The best transplanting time of the two peanut varieties was 7 days, and the fruit setting rates of ‘Laiyang Silihong’ and ‘83’ (‘Yunhua 24’) were 57.63% and 48.67%, respectively. In this study, the in vitro regeneration system of embryo leaflets of two peanut varieties, ‘Laiyang Silihong’ and ‘83’ (‘Yunhua 24’), was successfully established. The results not only improved the peanut tissue culture technology system, but also provided important transgenic technology platform support for subsequent peanut genetic improvement research and functional gene mining.

Key words: peanut, embryo leaflet buds, in vitro regeneration system, tissue culture method, plant growth regulators

CLC Number:

S565.2

LI Tingting, ZHANG Jiancheng, CHI Xiaoyuan, JIANG Xiao, LIU Xiaoqin. Arachis hypogaea L. Embryo Leaflet: Establishment and Optimization of in Vitro Regeneration System[J]. Chinese Agricultural Science Bulletin, 2026, 42(9): 145-154.

Figures/Tables 14

References 34

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处理编号	6-BA(mg/L)	NAA(mg/L)
A1	3	0.1
A2	3	0.2
A3	3	0.3
A4	3	0.4
A5	3.25	0.1
A6	3.25	0.2
A7	3.25	0.3
A8	3.25	0.4
A9	3.5	0.1
A10	3.5	0.2
A11	3.5	0.3
A12	3.5	0.4
A13	3.75	0.1
A14	3.75	0.2
A15	3.75	0.3
A16	3.75	0.4

处理编号	6-BA(mg/L)	NAA(mg/L)
A1	3	0.1
A2	3	0.2
A3	3	0.3
A4	3	0.4
A5	3.25	0.1
A6	3.25	0.2
A7	3.25	0.3
A8	3.25	0.4
A9	3.5	0.1
A10	3.5	0.2
A11	3.5	0.3
A12	3.5	0.4
A13	3.75	0.1
A14	3.75	0.2
A15	3.75	0.3
A16	3.75	0.4

处理编号	TDZ(mg/L)	6-BA(mg/L)	GA₃(mg/L)
B1	1(1)	2(1)	1(1)
B2	1(1)	3(2)	1.5(2)
B3	1(1)	4(3)	2(3)
B4	1.5(2)	2(1)	2(3)
B5	1.5(2)	3(2)	1(1)
B6	1.5(2)	4(3)	1.5(2)
B7	2(3)	2(1)	1.5(2)
B8	2(3)	3(2)	2(3)
B9	2(3)	4(3)	1(1)

处理编号	TDZ(mg/L)	6-BA(mg/L)	GA₃(mg/L)
B1	1(1)	2(1)	1(1)
B2	1(1)	3(2)	1.5(2)
B3	1(1)	4(3)	2(3)
B4	1.5(2)	2(1)	2(3)
B5	1.5(2)	3(2)	1(1)
B6	1.5(2)	4(3)	1.5(2)
B7	2(3)	2(1)	1.5(2)
B8	2(3)	3(2)	2(3)
B9	2(3)	4(3)	1(1)

处理编号	TDZ(mg/L)	6-BA(mg/L)	NAA(mg/L)	GA₃(mg/L)
C1	1(1)	3(1)	0.1(1)	1(1)
C2	1(1)	3.5(2)	0.3(2)	1.5(2)
C3	1(1)	4(3)	0.5(3)	2(3)
C4	1(1)	4.5(4)	1(4)	2.5(4)
C5	1.5(2)	3(1)	0.3(2)	2(3)
C6	1.5(2)	3.5(2)	0.1（1）	2.5(4)
C7	1.5(2)	4(3)	1(4)	1(1)
C8	1.5(2)	4.5(4)	0.5(3)	1.5(2)
C9	2(3)	3（1）	0.5(3)	2.5(4)
C10	2(3)	3.5(2)	1(4)	2(3)
C11	2(3)	4(3)	0.1(1)	1.5(2)
C12	2(3)	4.5(4)	0.3(2)	1(1)
C13	2.5(4)	3(1)	1(4)	1.5(2)
C14	2.5(4)	3.5(2)	0.5(3)	1(1)
C15	2.5(4)	4(3)	0.3(2)	2.5(4)
C16	2.5(4)	4.5(4)	0.1(1)	2(3)