Effects of Growth, Root Rot Disease, and Rhizosphere Microbial Community Structure of Panax notoginseng: A Combined Application of L-Lysine and Trichoderma harzianum

doi:10.11924/j.issn.1000-6850.casb2025-0135

Abstract

Abstract:

To investigate the synergistic control effect of exogenous substances on Panax notoginseng root rot disease caused by Fusarium solani, this study conducted in vitro plate culture method and outdoor pot experiments using combined application of L-Lysine and Trichoderma harzianum, aiming to clarify the effects of different treatments on F. solani strain, P. notoginseng seedling growth, root rot disease control, and their regulation on rhizosphere microbial diversity. The results showed that: 1 μmol/L L-Lysine exhibited only 13.32% inhibition rate against F. solani, but significantly promoted the mycelial growth (23.05%) and spore production (173.61%) of T. harzianum. In pot experiments, the LTF1 treatment (1 μmol/L L-Lysine+T. harzianum+F. solani) demonstrated the optimal application effect. Compared with CK (F. solani), the survival rate, root fresh weight and root dry weight of P. notoginseng increased by 45.83%, 27.45% and 33.33% respectively, while the root rot incidence decreased by 88.89%. High-throughput sequencing analysis of rhizosphere microbes revealed impacts on both fungal and bacterial diversity, with the most significant reduction observed in fungal community abundance. Ascomycota (87.27%) and Actinobacteriota (24.54%) emerged as dominant phyla, while functional genera including Trichoderma (28.57%), Anaeromyxobacter (2.47%) and Paenibacillus (1.96%) showed significant enrichment, exhibiting negative correlations with root rot incidence. This study provides new ecological management strategies for controlling P. notoginseng root rot disease through combined application of L-Lysine and Trichoderma harzianum.

Key words: L-Lysine, Trichoderma harzianum, Panax notoginseng root rot disease, high-throughput sequencing, rhizosphere microorganism

JIN Yongping, LIU Lianjin, REN Jing, FA Ze, LI Jinhua, WANG Huiling, WU Wentao, ZHU Youyong, HE Xiahong, GUO Liwei. Effects of Growth, Root Rot Disease, and Rhizosphere Microbial Community Structure of Panax notoginseng: A Combined Application of L-Lysine and Trichoderma harzianum[J]. Chinese Agricultural Science Bulletin, 2025, 41(17): 115-125.

Figures/Tables 13

References 38

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序号	试验处理	单次灌根用量/（mL/盆）	单次灌水量/（mL/盆）
CK	F. solani（茄腐镰刀菌）	50	150
TF	T. harzianum+F. solani（哈茨木霉菌+茄腐镰刀菌）	50+50	100
LF1	1 μmol/L L-Lysine+F. solani（L-赖氨酸+茄腐镰刀菌）	50+50	100
LF10	10 μmol/L L-Lysine+F. solani（L-赖氨酸+茄腐镰刀菌）	50+50	100
LF100	100 μmol/L L-Lysine+F. solani（L-赖氨酸+茄腐镰刀菌）	50+50	100
LTF1	1 μmol/L L-Lysine+T. harzianum+F. solani（L-赖氨酸+哈茨木霉菌+茄腐镰刀菌）	50+50+50	50
LTF10	10 μmol/L L-Lysine+T. harzianum+F. solani（L-赖氨酸+哈茨木霉菌+茄腐镰刀菌）	50+50+50	50
LTF100	100 μmol/L L-Lysine+T. harzianum+F. solani（L-赖氨酸+哈茨木霉菌+茄腐镰刀菌）	50+50+50	50

序号	试验处理	单次灌根用量/（mL/盆）	单次灌水量/（mL/盆）
CK	F. solani（茄腐镰刀菌）	50	150
TF	T. harzianum+F. solani（哈茨木霉菌+茄腐镰刀菌）	50+50	100
LF1	1 μmol/L L-Lysine+F. solani（L-赖氨酸+茄腐镰刀菌）	50+50	100
LF10	10 μmol/L L-Lysine+F. solani（L-赖氨酸+茄腐镰刀菌）	50+50	100
LF100	100 μmol/L L-Lysine+F. solani（L-赖氨酸+茄腐镰刀菌）	50+50	100
LTF1	1 μmol/L L-Lysine+T. harzianum+F. solani（L-赖氨酸+哈茨木霉菌+茄腐镰刀菌）	50+50+50	50
LTF10	10 μmol/L L-Lysine+T. harzianum+F. solani（L-赖氨酸+哈茨木霉菌+茄腐镰刀菌）	50+50+50	50
LTF100	100 μmol/L L-Lysine+T. harzianum+F. solani（L-赖氨酸+哈茨木霉菌+茄腐镰刀菌）	50+50+50	50

L-赖氨酸供试浓度/(μmol/L)	菌落直径/cm	抑制率/%	产孢量/(×10⁶CFU/mL)	孢子产生抑制率/%
0	4.47±0.10 a	—	0.26±0.02 b	—
1	3.86±0.06 b	13.32±1.7 a	0.41±0.02 a	-60.37±17.75 b
10	4.43±0.03 a	0.18±2.48 b	0.17±0.02 c	32.69±11.87 a
100	4.03±0.08 b	9.61±2.06 a	0.15±0.02 c	38.82±12.95 a

L-赖氨酸供试浓度/(μmol/L)	菌落直径/cm	抑制率/%	产孢量/(×10⁶CFU/mL)	孢子产生抑制率/%
0	4.47±0.10 a	—	0.26±0.02 b	—
1	3.86±0.06 b	13.32±1.7 a	0.41±0.02 a	-60.37±17.75 b
10	4.43±0.03 a	0.18±2.48 b	0.17±0.02 c	32.69±11.87 a
100	4.03±0.08 b	9.61±2.06 a	0.15±0.02 c	38.82±12.95 a

L-赖氨酸供试浓度/(μmol/L)	菌落直径/cm	促进率/%	产孢量/(×10⁶CFU/mL)	孢子产生促进率/%
0	7.00±0.08 c	—	40.00±4.62 b	—
1	8.61±0.15 a	23.05±2.19 a	106.67±6.67 a	173.61±32.84 a
10	8.03±0.15 b	14.79±2.49 b	86.67±12.02 a	118.06±22.35 a
100	7.93±0.06 b	13.49±1.55 b	116.67±17.64 a	202.08±58.00 a