Effects of Fluorescence and LED Light Sources on Growth of Haematococcus pluvialis

doi:10.11924/j.issn.1000-6850.casb2025-0341

Abstract

Abstract:

To study whether LED lights have more advantages in the cultivation of Haematococcus pluvialis, we selected two light sources, fluorescence and LED, and set three light intensity grades of 800 lx, 1000 lx and 1200 lx, respectively. During 10 days from inoculation to growth to stability period, the algae cell density and the growth rate were calculated every day, OD₆₈₀ and dry weigh were examined every two days. The results showed that, H. pluvialis in each group was in the latent period from day 1 to day 3 with maximum growth rate of 0.082, grew slowly from day 4 with growth rate of 0.110-0.232, and entered the exponential growth period on day 6 with growth rate of 0.301-0.662; from day 7, with the increase of light intensity of both light sources, the average cell density, OD₆₈₀, dry weight and average growth rate increased, while with the same light intensity, the 4 indicators in LED light sources were greater than that in fluorescent light sources, which were greater 8.82%-16.97% in 1200 lx on day 9. Therefore, the LED light sources are more beneficial for the growth of Haematococcus pluvialis compared to fluorescent light sources, significantly increasing algal cell density and biomass accumulation under a light intensity of 1200 lx. LED light sources are more advantageous in cultivation of H. pluvialis, besides of energy saving and low heat dissipation, which are recommended to use. This study provides a theoretical basis and data support for the large-scale cultivation of Haematococcus pluvialis using LED light sources to efficiently obtain high-value products like astaxanthin.

Key words: Haematococcus pluvialis, LEDs, fluorescent light sources, light intensity, light duration, feed microalgae

QI Jianfei, WANG Jun, WU Qisheng, ZHOU Xiaowen, YUAN Zehui, NING Yue, ZOU Shuangyan, JIA Yuanyuan. Effects of Fluorescence and LED Light Sources on Growth of Haematococcus pluvialis[J]. Chinese Agricultural Science Bulletin, 2025, 41(32): 158-164.

Figures/Tables 8

References 35

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日期	OD₆₈₀			干重/(mg/L)
日期	800 lx	1000 lx	1200 lx	800 lx	1000 lx	1200 lx
第1 d	0.043	0.043	0.045	40	40	40
第3 d	0.052	0.052	0.053	60	60	60
第5 d	0.057	0.059	0.060	60	70	70
第7 d	0.121	0.110	0.136	130	120	150
第9 d	0.223	0.249	0.307	190	210	260

日期	OD₆₈₀			干重/(mg/L)
日期	800 lx	1000 lx	1200 lx	800 lx	1000 lx	1200 lx
第1 d	0.043	0.043	0.045	40	40	40
第3 d	0.052	0.052	0.053	60	60	60
第5 d	0.057	0.059	0.060	60	70	70
第7 d	0.121	0.110	0.136	130	120	150
第9 d	0.223	0.249	0.307	190	210	260

光照强度/lx	平均生长速率/d		平均藻密度（×10⁴个/mL）
光照强度/lx	荧光	LED	荧光	LED
800	0.164	0.217	10.573	13.627
1000	0.177	0.222	10.996	14.520
1200	0.206	0.223	13.357	14.843

光照强度/lx	平均生长速率/d		平均藻密度（×10⁴个/mL）
光照强度/lx	荧光	LED	荧光	LED
800	0.164	0.217	10.573	13.627
1000	0.177	0.222	10.996	14.520
1200	0.206	0.223	13.357	14.843

日期	OD₆₈₀			干重/(mg/L)
日期	800 lx	1000 lx	1200 lx	800 lx	1000 lx	1200 lx
第1 d	0.045	0.046	0.046	40	40	40
第3 d	0.054	0.056	0.057	60	70	70
第5 d	0.055	0.059	0.059	60	70	70
第7 d	0.13	0.125	0.134	140	130	140
第9 d	0.32	0.356	0.358	270	300	300