A Method of Sugar Free Tissue Culture and Rapid Propagation of Virus-free Potato Seedlings

doi:10.11924/j.issn.1000-6850.casb2023-0074

Abstract

Abstract:

In order to improve the quality of virus-free potato seedlings and optimize potato seedlings production, the application of sugar free culture in rapid propagation of virus-free potato seedlings was studied. The virus-free plantlets of potato early maturing variety ‘Atlantic’ were used as materials. The results showed that after 25 days of sugar free culture, the plant height, internode number, and rootless fresh quality of potato seedlings double stage inoculation were significantly greater than those inoculated in single segment, double stage inoculation was more suitable for rapid propagation of virus-free potato seedlings. The segments of potato virus-free seedlings grew best in a sugar free culture box supported by plastic particles, but the price was relatively expensive. Therefore, it was recommended to use vermiculite as the support for comprehensive consideration. The plant height of potato plantlets injected with CO₂ 3 days after inoculation was 10.29 cm, significantly higher than that of other treatments. Under the conditions of light intensity of 8000-8500 lx and CO₂ concentration of 1×10⁶ mg/m³ sugar-free culture, potato virus-free seedlings had the best growth potential, with a plant height of 10.28 cm, stem diameter of 1.55 cm, and fresh weight of 0.90 g, significantly higher than other treatments.

Key words: potato, virus-free seedling, sugar free tissue culture, CO₂ concentration, light intensity

LIU Dan, LIANG Ruiping, LI Xiuhua, WANG Liangming, BAI Yinfeng. A Method of Sugar Free Tissue Culture and Rapid Propagation of Virus-free Potato Seedlings[J]. Chinese Agricultural Science Bulletin, 2023, 39(22): 11-15.

Figures/Tables 6

References 25

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节段	株高/cm	茎粗/mm	节间数	鲜质量/g
双节段	10.3c	1.59bc	4.91b	0.61c
单节段	8.81b	1.34b	4.73b	0.55b
对照	5.9a	0.56a	3.78a	0.26a

节段	株高/cm	茎粗/mm	节间数	鲜质量/g
双节段	10.3c	1.59bc	4.91b	0.61c
单节段	8.81b	1.34b	4.73b	0.55b
对照	5.9a	0.56a	3.78a	0.26a

通入CO₂时间/d	株高/cm	茎粗/mm	节间数	鲜质量/g
0	8.07b	1.04b	4.95b	0.35c
3	10.29b	1.13b	5.05b	0.43d
5	8.31b	1.12b	4.93b	0.33bc
7	8.8b	1.03b	4.65b	0.29a
对照	5.9a	0.56a	3.78a	0.26ab

通入CO₂时间/d	株高/cm	茎粗/mm	节间数	鲜质量/g
0	8.07b	1.04b	4.95b	0.35c
3	10.29b	1.13b	5.05b	0.43d
5	8.31b	1.12b	4.93b	0.33bc
7	8.8b	1.03b	4.65b	0.29a
对照	5.9a	0.56a	3.78a	0.26ab

支撑物	株高/cm	茎粗/cm	节间数	鲜质量/g
蛭石	8.68b	0.54a	6.22a	0.27b
珍珠岩	7.13a	0.54a	5.36ab	0.13a
塑料颗粒	9.42b	0.83b	6.40b	0.33b
卡拉胶	9.03b	0.96c	6.42b	0.29b
对照	5.9a	0.56a	3.78a	0.26ab