Indoor Fungicide Screening for Two Strains of Fusarium Spp. with Strong Pathogenicity to Sweet Potato

doi:10.11924/j.issn.1000-6850.casb2022-0251

Abstract

Abstract:

Fusarium solani-melongenae and Fusarium oxysporum have strong pathogenicity to sweet patato. To screen effective fungicides to control them, the indoor toxicity of eight fungicides to the two strains of Fusarium spp. pathogens were detected by using mycelial growth rate method, and the eight fungicides were carbendazim, thiophanate-methyl, procymidone, trifloxystrobin·tebuconazole, pyraclostrobin·metiram, prochloraz, zhongshengmycin and tetramycin. The results showed that tetramycin and prochloraz were found to be the most effective fungicides inhibiting the mycelial growth of Fusarium solani-melongenae, and the mean effective concentration (EC₅₀) value was 1.292 μg/mL and 2.145 μg/mL, respectively. The EC₅₀ value of zhongshengmycin and trifloxystrobin·tebuconazole was 16.652 μg/mL and 28.641 μg/mL respectively, also effective against Fusarium solani-melongenae. However, procymidone, thiophanate-methyl, carbendazim and pyraclostrobin·metiram, with EC₅₀ value of 440.540, 637.120, 680.765 and 22385.367 μg/mL respectively, were less effective against Fusarium solani-melongenae. Tetramycin, prochloraz and trifloxystrobin·tebuconazole were the most effective fungicides inhibiting the mycelial growth of Fusarium oxysporum, and the mean effective concentration (EC₅₀) value was 0.03, 0.131 and 0.177 μg/mL, respectively. Zhongshengmycin and carbendazim were also effective against Fusarium oxysporum, and the EC₅₀ value was 3.66 μg/mL and 14.408 μg/mL, respectively. Thiophanate-methyl and pyraclostrobin·metiram had poor inhibitory effect on the mycelial growth of Fusarium oxysporum strains, with EC₅₀ of 28.611 μg/mL and 59.78 μg/mL, respectively. The inhibitory effect of procymidone was the worst, its EC₅₀ value was 379.426 μg/mL. Overall, tetramycin and prochloraz could obviously inhibit the two strains of Fusarium spp., and there were significant differences of the sensitivity of the two strains of Fusarium spp. to different fungicides. The sensitivity of Fusarium oxysporum to the eight fungicides was higher than that of Fusarium solani-melongenae.

Key words: sweet potato, Fusarium solani-melongenae, Fusarium oxysporum, toxicity test

CHEN Xinliang, CHEN Jingyi, LUO Zhongxia, WANG Zhangying, FANG Boping, WANG Xiaobin, HUANG Lifei. Indoor Fungicide Screening for Two Strains of Fusarium Spp. with Strong Pathogenicity to Sweet Potato[J]. Chinese Agricultural Science Bulletin, 2023, 39(8): 106-111.

Figures/Tables 4

References 25

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药剂	浓度/(µg/mL)	菌落直径/cm	抑菌率/%
对照	0	7	0
50%多菌灵	1280	2.3	72.3
	320	5.2	27.7
	80	6.5	7.7
	20	6.6	6.2
	5	6.7	4.6
70%甲基硫菌灵	1280	2	76.9
	320	5.5	23.1
	80	6.4	9.2
	20	6.5	7.7
	5	6.7	4.6
50%腐霉利	600	2.5	69.2
	500	3.4	55.4
	300	5.4	24.6
	100	6.6	6.2
	80	6.7	4.6
75%肟菌戊唑醇	160	2.5	69.2
	80	3.3	56.9
	40	3.6	52.3
	20	3.9	47.7
	10	4.4	40.0
60%唑醚代森联	1600	4.5	38.5
	400	4.8	33.8
	100	5	30.8
	25	5.3	26.2
	6.25	5.8	18.5
45%咪鲜胺	24.3	2	76.9
	8.1	2.7	66.2
	2.7	3.6	52.3
	0.9	4.4	40.0
	0.3	5.2	27.7
	0.1	5.9	16.9
3%中生菌素	80	2.5	69.2
	40	2.8	64.6
	20	3.3	56.9
	10	4.3	41.5
	5	5	30.8
0.3%四霉素	20	0.6	98.5
	10	0.6	98.5
	5	0.6	98.5
	2.5	3	61.5
	1.25	3.5	53.8

药剂	浓度/(µg/mL)	菌落直径/cm	抑菌率/%
对照	0	7	0
50%多菌灵	1280	2.3	72.3
	320	5.2	27.7
	80	6.5	7.7
	20	6.6	6.2
	5	6.7	4.6
70%甲基硫菌灵	1280	2	76.9
	320	5.5	23.1
	80	6.4	9.2
	20	6.5	7.7
	5	6.7	4.6
50%腐霉利	600	2.5	69.2
	500	3.4	55.4
	300	5.4	24.6
	100	6.6	6.2
	80	6.7	4.6
75%肟菌戊唑醇	160	2.5	69.2
	80	3.3	56.9
	40	3.6	52.3
	20	3.9	47.7
	10	4.4	40.0
60%唑醚代森联	1600	4.5	38.5
	400	4.8	33.8
	100	5	30.8
	25	5.3	26.2
	6.25	5.8	18.5
45%咪鲜胺	24.3	2	76.9
	8.1	2.7	66.2
	2.7	3.6	52.3
	0.9	4.4	40.0
	0.3	5.2	27.7
	0.1	5.9	16.9
3%中生菌素	80	2.5	69.2
	40	2.8	64.6
	20	3.3	56.9
	10	4.3	41.5
	5	5	30.8
0.3%四霉素	20	0.6	98.5
	10	0.6	98.5
	5	0.6	98.5
	2.5	3	61.5
	1.25	3.5	53.8

药剂	浓度/(µg/mL)	菌落直径/cm	抑菌率/%
对照	0	7.4	0
50%多菌灵	800	2.5	71.0
	400	2.6	69.6
	200	2.8	66.7
	100	3.2	60.9
	50	3.5	56.5
70%甲基硫菌灵	800	1.8	81.2
	400	2.4	72.5
	200	2.7	68.1
	100	3.3	59.4
	50	3.4	58.0
50%腐酶利	1000	1.2	89.9
	800	1.6	84.1
	600	2.3	73.9
	400	3.7	53.6
	200	6.1	18.8
75%肟菌戊唑醇	160	1.9	79.7
	80	2	78.3
	40	2.2	75.4
	20	2.3	73.9
	10	2.7	68.1
60%唑醚代森联	800	2.6	69.6
	400	2.9	65.2
	200	3.2	60.9
	100	3.8	52.2
	50	4	49.3
45%咪鲜胺	12	1.3	88.4
	6	1.4	87.0
	3	1.7	82.6
	1.5	2	78.3
	0.75	2.6	69.6
	0.375	3.3	59.4
3%中生菌素	80	1.7	82.6
	40	2.2	75.4
	20	2.4	72.5
	10	3.2	60.9
	5	3.7	53.6
0.3%四霉素	2.4	1.4	87.0
	1.2	2	78.3
	0.6	2.1	76.8
	0.3	2.3	73.9
	0.15	3	63.8

药剂	浓度/(µg/mL)	菌落直径/cm	抑菌率/%
对照	0	7.4	0
50%多菌灵	800	2.5	71.0
	400	2.6	69.6
	200	2.8	66.7
	100	3.2	60.9
	50	3.5	56.5
70%甲基硫菌灵	800	1.8	81.2
	400	2.4	72.5
	200	2.7	68.1
	100	3.3	59.4
	50	3.4	58.0
50%腐酶利	1000	1.2	89.9
	800	1.6	84.1
	600	2.3	73.9
	400	3.7	53.6
	200	6.1	18.8
75%肟菌戊唑醇	160	1.9	79.7
	80	2	78.3
	40	2.2	75.4
	20	2.3	73.9
	10	2.7	68.1
60%唑醚代森联	800	2.6	69.6
	400	2.9	65.2
	200	3.2	60.9
	100	3.8	52.2
	50	4	49.3
45%咪鲜胺	12	1.3	88.4
	6	1.4	87.0
	3	1.7	82.6
	1.5	2	78.3
	0.75	2.6	69.6
	0.375	3.3	59.4
3%中生菌素	80	1.7	82.6
	40	2.2	75.4
	20	2.4	72.5
	10	3.2	60.9
	5	3.7	53.6
0.3%四霉素	2.4	1.4	87.0
	1.2	2	78.3
	0.6	2.1	76.8
	0.3	2.3	73.9
	0.15	3	63.8

药剂	CRI-FS28			CRI-FO20
药剂	回归方程	相关系数r	EC₅₀(95% FL)/(μg/mL)	回归方程	相关系数r	EC₅₀(95%FL)/(μg/mL)
50%多菌灵	y=0.91x-2.67	0.837	680.765(165.595~1.41×10⁶)	y=0.34x-0.39	0.959	14.408(0.001~52.003)
70%甲基硫菌灵	y=0.92x-2.64	0.810	637.120(119.413~9.21×10⁷)	y=0.57x-0.84	0.949	28.611(4.364~59.126)
50%腐酶利	y=2.4x-6.34	0.968	440.540(332.848~652.949)	y=3.1x-8	0.999	379.426(338.926~418.101)
75%肟菌·戊唑醇	y=0.58x-0.84	0.957	28.641(15.436~44.881)	y=0.29x+0.22	0.946	0.177(0~2.716)
60%唑醚·代森联	y=0.4x-1.02	0.953	22385.367(2566.509~6.00×10⁷)	y=0.46x-0.82	0.980	59.78(2.605~70.978)
45%咪鲜胺	y=0.71x-0.24	1	2.145(1.515~3.096)	y=0.64x+0.59	0.966	0.131(0.032~0.275)
3%中生菌素	y=0.86x-1.05	0.966	16.652(11.858~22.544)	y=0.7x-0.39	0.980	3.66(1.152~6.410)
0.3%四霉素	y=2x+0.02	0.772	1.292(0.001~2.488)	y=0.56x+0.85	0.920	0.03(0.001~0.087)