Secondary Metabolites Extracted From Sixteen Plant Species: Antibacterial Effects on Two Strains of Plant Pathogenic Bacteria

doi:10.11924/j.issn.1000-6850.casb2022-0195

Abstract

Abstract:

The study aims to search and develop environment-friendly and effective Chinese herbal medicine resources to inhibit plant pathogenic fungi, and to provide a research basis for the development of plant-derived fungicides. In this study, the fungicidal activity of 16 ethanol extracts from Ligusticum chuanxiong Hort. ex S.H. Qiu & al., Phellodendron amurense Rupr., Forsythia suspense (Thunb.)Vahl, and Euphorbia humifusa Willd. ex Schltdl. were determined, and the effects of petroleum ether extract of Ligusticum chuanxiong Hort. ex S.H. Qiu & al., which had the best effect on mycelial growth inhibition, on the spore germination and mycelial morphology of Fusarium oxysporum f. sp. Cucumerinum. and Alternaria solani (Ell.et Mart.) Jones et Grout. fungus were observed. The results showed that the petroleum ether extract of Ligusticum chuanxiong Hort. ex S.H. Qiu & al., ethyl acetate extract of Forsythia suspense (Thunb.) Vahl and n-butanol extract of Phellodendron amurense Rupr. had good inhibitory activities on the mycelial growth of the two pathogens. Among them, the intermediate inhibitory concentration (IC₅₀) of Ligusticum chuanxiong Hort. ex S.H. Qiu & al. petroleum ether extract on the mycelial growth of Fusarium oxysporum f. sp. Cucumerinum. and Alternaria solani (Ell.et Mart.) Jones et Grout. was 3.68 mg/mL and 5.82 mg/mL, respectively; Ligusticum chuanxiong Hort. ex S.H. Qiu & al. petroleum ether extract also inhibited the spore germination of Fusarium oxysporum f. sp. Cucumerinum with an IC₅₀ of 11.29 mg/mL, and caused abnormal mycelial morphology at the IC₅₀ concentration of mycelial growth. The petroleum ether extract of Ligusticum chuanxiong Hort. ex S.H. Qiu & al. can be used as a new source of plant-derived inhibition agent for further study.

Key words: plant extract, Ligusticum chuanxiong., Fusarium oxysporum f. sp. Cucumerinum., antifungal activity

WANG Xin, SHEN Yalun, CHEN Sibo, LI Yunpeng, LIU Jianan. Secondary Metabolites Extracted From Sixteen Plant Species: Antibacterial Effects on Two Strains of Plant Pathogenic Bacteria[J]. Chinese Agricultural Science Bulletin, 2023, 39(6): 129-134.

Figures/Tables 6

References 27

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中草药	萃取物	黄瓜枯萎病菌	番茄早疫病菌
川芎	PE	(82.72±0.67)a(a)	(63.46±1.69)a(b)
	AE	(37.91±0.46)b	(24.03±1.68)d
	BE	(7.78±0.94)def	(4.12±0.79)fg
	WE	(-14.29±0.67)h	(-5.69±1.69)i
黄柏	PE	(1.67±1.09)efg	(6.84±1.15)f
	AE	(-12.95±0.81)h	(3.11±1.49)fg
	BE	(37.84±0.93)b(a)	(33.47±0.84)c(b)
	WE	(-2.65±1.80)efgh	(6.64±0.80)f
连翘	PE	(19.19±1.68)cd	(21.88±1.42)d
	AE	(33.75±1.59)bc(b)	(48.10±0.89)b(a)
	BE	(10.19±1.20)de	(5.87±1.45)f
	WE	(-14.38±1.15)h	(-0.43±0.70)gh
地锦草	PE	(2.85±0.28)efg	(-2.16±0.99)hi
	AE	(-4.17±0.45)fgh	(7.58±1.10)f
	BE	(-10.27±0.50)h	(12.69±1.40)e
	WE	(-5.88±1.26)h	(-4.13±1.57)hi

中草药	萃取物	黄瓜枯萎病菌	番茄早疫病菌
川芎	PE	(82.72±0.67)a(a)	(63.46±1.69)a(b)
	AE	(37.91±0.46)b	(24.03±1.68)d
	BE	(7.78±0.94)def	(4.12±0.79)fg
	WE	(-14.29±0.67)h	(-5.69±1.69)i
黄柏	PE	(1.67±1.09)efg	(6.84±1.15)f
	AE	(-12.95±0.81)h	(3.11±1.49)fg
	BE	(37.84±0.93)b(a)	(33.47±0.84)c(b)
	WE	(-2.65±1.80)efgh	(6.64±0.80)f
连翘	PE	(19.19±1.68)cd	(21.88±1.42)d
	AE	(33.75±1.59)bc(b)	(48.10±0.89)b(a)
	BE	(10.19±1.20)de	(5.87±1.45)f
	WE	(-14.38±1.15)h	(-0.43±0.70)gh
地锦草	PE	(2.85±0.28)efg	(-2.16±0.99)hi
	AE	(-4.17±0.45)fgh	(7.58±1.10)f
	BE	(-10.27±0.50)h	(12.69±1.40)e
	WE	(-5.88±1.26)h	(-4.13±1.57)hi

病原菌种类	中草药萃取物	5 mg/mL	10 mg/mL	20 mg/mL	30 mg/mL	40 mg/mL
黄瓜枯萎病菌	川芎石油醚相	(54.70±0.39)d(a)	(77.79±0.14)c(a)	(88.39±0.87)b(a)	(90.03±1.42)b(a)	(94.18±1.35)a(a)
	连翘乙酸乙酯相	(18.77±1.22)d	(35.83±2.25)c	(53.86±3.11)b	(66.63±2.00)a	(67.86±1.71)a
	黄柏正丁醇相	(4.78±0.68)d	(18.73±1.80)c	(34.47±1.47)b	(37.49±0.69)b	(46.99±2.17)a
番茄早疫病菌	川芎石油醚相	(45.33±1.25)e(b)	(65.69±0.22)d(b)	(84.65±0.52)c(a)	(89.54±0.81)b(a)	(93.48±0.25)a(a)
	连翘乙酸乙酯相	(25.19±0.21)e	(32.12±0.15)d	(41.53±1.79)c	(50.24±0.98)b	(55.76±1.53)a
	黄柏正丁醇相	(12.19±0.52)e	(27.34±0.57)d	(34.62±0.67)c	(40.12±0.75)b	(54.45±0.81)a

病原菌种类	中草药萃取物	5 mg/mL	10 mg/mL	20 mg/mL	30 mg/mL	40 mg/mL
黄瓜枯萎病菌	川芎石油醚相	(54.70±0.39)d(a)	(77.79±0.14)c(a)	(88.39±0.87)b(a)	(90.03±1.42)b(a)	(94.18±1.35)a(a)
	连翘乙酸乙酯相	(18.77±1.22)d	(35.83±2.25)c	(53.86±3.11)b	(66.63±2.00)a	(67.86±1.71)a
	黄柏正丁醇相	(4.78±0.68)d	(18.73±1.80)c	(34.47±1.47)b	(37.49±0.69)b	(46.99±2.17)a
番茄早疫病菌	川芎石油醚相	(45.33±1.25)e(b)	(65.69±0.22)d(b)	(84.65±0.52)c(a)	(89.54±0.81)b(a)	(93.48±0.25)a(a)
	连翘乙酸乙酯相	(25.19±0.21)e	(32.12±0.15)d	(41.53±1.79)c	(50.24±0.98)b	(55.76±1.53)a
	黄柏正丁醇相	(12.19±0.52)e	(27.34±0.57)d	(34.62±0.67)c	(40.12±0.75)b	(54.45±0.81)a

病原菌种类	中草药萃取物	毒力回归方程	相关系数	IC₅₀（95%置信限）(mg/mL)
黄瓜枯萎病菌	川芎石油醚相	y=4.1409+1.5177x	0.9864	3.68(2.68~5.05)
	连翘乙酸乙酯相	y=3.0605+1.5526x	0.9944	17.75(16.20~19.45)
	黄柏正丁醇相	y=2.2832+1.6837x	0.9796	41.07(31.18~54.11)
番茄早疫病菌	川芎石油醚相	y=3.6187+1.8066x	0.9988	5.82(5.42~6.24)
	连翘乙酸乙酯相	y=3.6313+0.9375x	0.9882	28.84(24.45~34.02)
	黄柏正丁醇相	y=3.0120+1.2564x	0.9756	38.22(28.64~50.99)