Screening, Identification and Functional Determination of Endophytic Bacteria from Forage Grass at Alpine Grassland in Qinghai Province

doi:10.11924/j.issn.1000-6850.casb2022-0185

Abstract

Abstract:

The antagonistic strains against Fusarium oxysporum f.sp.cucumerium were screened from endophytic bacteria of forage grass at alpine grassland in Qinghai Province, and then were identified by morphological characteristics and homology analysis of 16Sr DNA gene sequence, and their biological functions were determined. The results showed that 36 strains of the 81 tested endophytic bacteria strains had antagonistic effects, accounting for 44.0%, and the inhibition rate of 13 strains isolated from the root were all above 50.0%, among which the inhibition rate of 2018GH11 was above 50.0% against 6 genera 8 species of pathogenic fungi, and 67.6% against Fusarium oxysporum. 2018GH11 had a broad spectrum of inhibition, and it was identified as Bacillus siamese by the morphological characteristics and homology analysis of 16Sr DNA gene sequence. 2018GH11 had nitrogen fixation ability, but the ability to produce IAA and solubilize phosphate was not obvious. In pot experiment, when the amount of 2018GH11 was 0.50%, the cucumber height, stem diameter, leaf number, fresh weight and dry weight were increased by 15.33%, 39.40%, 22.22%, 215.99% and 126.70%, respectively, compared with those of the control plants. In summary, the endophytic bacteria 2018GH11 from alpine grassland has a good effect on controlling disease and promoting plant growth, and a great potential for development.

Key words: Fusarium oxysporum f.sp.cucumerium, endophytic bacteria, antagonism, screening, identification

YAO Hemin, TANG Mei, GONG Xuehua, JIN Mengjun, YANG Chengde. Screening, Identification and Functional Determination of Endophytic Bacteria from Forage Grass at Alpine Grassland in Qinghai Province[J]. Chinese Agricultural Science Bulletin, 2023, 39(6): 124-128.

Figures/Tables 5

References 22

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菌株	抑菌率	菌株	抑菌率
2018GH11	57.53	2018G5	54.37
2018G9	57.51	2018G13	54.33
2018G7	57.49	2018G4	54.33
2018G3	56.16	2018G7	53.85
2018G12	56.16	2018G2	53.63
2018G3	55.70	2018G4	51.60
2018G5	55.70	-	-

菌株	抑菌率	菌株	抑菌率
2018GH11	57.53	2018G5	54.37
2018G9	57.51	2018G13	54.33
2018G7	57.49	2018G4	54.33
2018G3	56.16	2018G7	53.85
2018G12	56.16	2018G2	53.63
2018G3	55.70	2018G4	51.60
2018G5	55.70	-	-

病菌	2018GH11抑菌率
番茄早疫病菌Alternaria solani	61.6
小麦根腐病菌Bipolaris sorokiniana	64.4
马铃薯黑痣病菌Rhizoctonia solani	51.8
孜然根腐病菌Fusarium oxysporum	67.6
番瓜根腐病菌Fusarium sp.	60
马铃薯坏疽病菌Phoma foveata	64.7
马铃薯枯萎病菌Fusarium avenaceum	63.6

病菌	2018GH11抑菌率
番茄早疫病菌Alternaria solani	61.6
小麦根腐病菌Bipolaris sorokiniana	64.4
马铃薯黑痣病菌Rhizoctonia solani	51.8
孜然根腐病菌Fusarium oxysporum	67.6
番瓜根腐病菌Fusarium sp.	60
马铃薯坏疽病菌Phoma foveata	64.7
马铃薯枯萎病菌Fusarium avenaceum	63.6

处理	株高/cm	茎粗/cm	叶片数/个	鲜重/g	干重/g
0%	168.50±1.28^ab	0.33±0.04^ab	9±1.53^ab	22.58±0.85^b	3.67±1.89^b
0.50%	194.33±3.56^a	0.46±0.07^a	11±1.33^a	71.35±1.69^a	8.32±2.29^a
1%	146.00±1.57^bc	0.21±0.06^b	8±0.58^ab	44.31±1.05^ab	2.71±1.18^b
5%	120.67±2.56^c	0.39±0.09^ab	7±1.73^b	35.49±1.59^ab	4.34±1.01^ab