Blister Blight Lesions of Tea (Camellia sinensis L. Kuntze) Leaves: Microbial Diversity Analysis and Identification of the Disease Fungi

doi:10.11924/j.issn.1000-6850.casb2022-0348

Abstract

Abstract:

The aims are to analyze the microbial diversity on the surface of tea blister blight diseased leaves, and isolate and identify fungal microorganisms on diseased tea leaves. Tea leaves infected with blister blight disease and healthy leaves were used as test materials, and the leaf surface was observed by scanning electron microscopy, and the bacterial and fungal community compositions of different leaves were analyzed by 16S and ITS sequencing. 113 kinds of bacteria and 252 kinds of fungi were detected on the surface of tea blister blight diseased leaves, and 237 kinds of bacteria and 161 kinds of fungi were detected on healthy leaves. The bacterial diversity of diseased leaves was significantly lower than that of healthy leaves, and the fungi diversity of diseased leaves was higher than that of healthy leaves. Compared with healthy leaves, the abundance of Cladosporium and Pestalotiopsis in diseased leaves was lower, while that of Mortierella was higher. Fungal microorganism Cladosporium cladosporioides was isolated by PDA medium and identified, which might play an important role in the infection of blister blight disease. The microbial community structure on the surface of tea leaves changed significantly after tea blister blight disease infection, and the isolated and identified Cladosporium cladosporium might be closely related to the pathogenic process of tea blister blight disease.

Key words: blister blight, Camellia sinensis, microbial diversity, bacteria, fungi

CHEN Yiyong, ZHOU Bo, LI Jianlong, TANG Hao, CUI Yingying, WU Wenhao, LIU Jiayu, TANG Jinchi. Blister Blight Lesions of Tea (Camellia sinensis L. Kuntze) Leaves: Microbial Diversity Analysis and Identification of the Disease Fungi[J]. Chinese Agricultural Science Bulletin, 2023, 39(6): 116-123.

Figures/Tables 5

References 25

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基因	引物名称	引物序列(5’-3’)
ITS	ITS1	TCCGTAGGTGAACCTGCGG
ITS	ITS4	TCCTCCGCTTATTGATATGC
β-tubulin	Bt-2a-F	GGTAACCAAATCGGTGCTGCTTTC
β-tubulin	Bt-2a-R	ACCCTCAGTGTAGTGACCCTTGGC
Large subunit	LUS-F	GCATTCAATAAGCGGAGGAAAAG
Large subunit	LUS-R	GGTCCGTGTTTCAAGACGG

基因	引物名称	引物序列(5’-3’)
ITS	ITS1	TCCGTAGGTGAACCTGCGG
ITS	ITS4	TCCTCCGCTTATTGATATGC
β-tubulin	Bt-2a-F	GGTAACCAAATCGGTGCTGCTTTC
β-tubulin	Bt-2a-R	ACCCTCAGTGTAGTGACCCTTGGC
Large subunit	LUS-F	GCATTCAATAAGCGGAGGAAAAG
Large subunit	LUS-R	GGTCCGTGTTTCAAGACGG

比较项目	叶片类型	Shannon	Chao1	物种数观测值	PD整树
细菌16S	感病叶片	1.64 ± 0.27	200.04 ± 6.73	113 ± 9	12.15 ± 0.44
细菌16S	健康叶片	6.25 ± 0.12	264.63 ± 17.30	237 ± 11	21.57 ± 1.76
真菌ITS	感病叶片	2.39 ±0.70	287.48 ± 80.40	252 ± 90	69.29 ± 18.51
真菌ITS	健康叶片	1.52 ± 0.43	198.51 ± 60.70	161 ± 51	62.13 ± 12.82

比较项目	叶片类型	Shannon	Chao1	物种数观测值	PD整树
细菌16S	感病叶片	1.64 ± 0.27	200.04 ± 6.73	113 ± 9	12.15 ± 0.44
细菌16S	健康叶片	6.25 ± 0.12	264.63 ± 17.30	237 ± 11	21.57 ± 1.76
真菌ITS	感病叶片	2.39 ±0.70	287.48 ± 80.40	252 ± 90	69.29 ± 18.51
真菌ITS	健康叶片	1.52 ± 0.43	198.51 ± 60.70	161 ± 51	62.13 ± 12.82