Optimization of Culture Medium for Uric Acid-reducing Bacterial Strain

doi:10.11924/j.issn.1000-6850.casb2025-0650

Abstract

Abstract:

This study aimed to enhance the biomass content of Lactobacillus paracasei and provide technical support for its application in the prevention or treatment of hyperuricemia. The culture medium for Lactobacillus paracasei was optimized through single-factor and response surface methodology (RSM) experiments. Based on the screening of carbon sources, nitrogen sources, buffer salts, and nutritional factors via single-factor experiments, a Box-Behnken response surface design was employed. The results showed that a multiple quadratic regression model was established with OD₆₀₀ of Lactobacillus paracasei as the response value. Analysis of the model indicated that the optimal culture medium composition for Lactobacillus paracasei was as follows: soluble starch 35.1 g/L, yeast extract 45.6 g/L, disodium hydrogen phosphate 7.3 g/L, and white radish juice 82.8 g/L. After optimization, the viable count of Lactobacillus paracasei cultured in the optimized medium reached 6.16×10⁹ cfu/mL, which was 3.2 times higher than before optimization.

Key words: single-factor experiment, response surface methodology, Lactobacillus paracasei, Box-Behnken, optimization, optimal, culture medium, viable count

CLC Number:

S-3农业科学研究、试验

PAN Dongmei, LIU Zhenxue, YANG Chuanlun, SU Shigang, PAN Yunxia, LIU Jielei, WANG Xuliang, MA Nana, CAI Qianqian. Optimization of Culture Medium for Uric Acid-reducing Bacterial Strain[J]. Chinese Agricultural Science Bulletin, 2026, 42(17): 42-48.

Figures/Tables 12

References 25

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方差来源	平方和	自由度	均方	F值	P值	显著性
模型	2.86	14	0.20	24.27	<0.0001	极显著
A	0.38	1	0.38	44.71	<0.0001	极显著
B	0.42	1	0.42	49.46	<0.0001	极显著
C	0.085	1	0.085	10.05	0.0068	极显著
D	0.093	1	0.093	11.03	0.0050	极显著
AB	0.050	1	0.050	5.91	0.0290	显著
AC	3.192E-003	1	3.192E-003	0.38	0.5477	不显著
AD	0.015	1	0.015	1.73	0.2100	不显著
BC	0.014	1	0.014	1.63	0.2228	不显著
BD	0.024	1	0.024	2.89	0.1110	不显著
CD	0.052	1	0.052	6.24	0.0256	显著
A²	0.56	1	0.56	66.69	<0.0001	极显著
B²	0.65	1	0.65	77.53	<0.0001	极显著
C²	0.49	1	0.49	58.72	<0.0001	极显著
D²	0.96	1	0.96	114.72	<0.0001	极显著
残差	0.12	14	8.409E-003	—	—	—
失拟项	0.10	10	0.010	2.60	0.1854	不显著
纯误差	0.016	4	3.929E-003	—	—	—
总离差	2.98	28	—	—	—	—

方差来源	平方和	自由度	均方	F值	P值	显著性
模型	2.86	14	0.20	24.27	<0.0001	极显著
A	0.38	1	0.38	44.71	<0.0001	极显著
B	0.42	1	0.42	49.46	<0.0001	极显著
C	0.085	1	0.085	10.05	0.0068	极显著
D	0.093	1	0.093	11.03	0.0050	极显著
AB	0.050	1	0.050	5.91	0.0290	显著
AC	3.192E-003	1	3.192E-003	0.38	0.5477	不显著
AD	0.015	1	0.015	1.73	0.2100	不显著
BC	0.014	1	0.014	1.63	0.2228	不显著
BD	0.024	1	0.024	2.89	0.1110	不显著
CD	0.052	1	0.052	6.24	0.0256	显著
A²	0.56	1	0.56	66.69	<0.0001	极显著
B²	0.65	1	0.65	77.53	<0.0001	极显著
C²	0.49	1	0.49	58.72	<0.0001	极显著
D²	0.96	1	0.96	114.72	<0.0001	极显著
残差	0.12	14	8.409E-003	—	—	—
失拟项	0.10	10	0.010	2.60	0.1854	不显著
纯误差	0.016	4	3.929E-003	—	—	—
总离差	2.98	28	—	—	—	—