Isolation and Identification of Pathogen Causing Black Mold Disease on Ginger During Storage and Study on Its Biological Characteristics

doi:10.11924/j.issn.1000-6850.casb2025-0748

Abstract

Abstract:

This study aimed to identify the pathogen causing black mold on stored ginger in Sichuan and clarify its key biological characteristics, so as to provide a theoretical basis for green control strategies. The pathogenic strain CDSJ1 was obtained by tissue isolation, and identified based on morphological characteristics, molecular biology (ITS sequence analysis), and verification using Koch’s postulates. The effects of temperature, carbon source, nitrogen source, and light conditions on mycelial growth and sporulation were investigated through single-factor experiments. The pathogen was identified as Aspergillus niger. The optimal growth temperature was 30 ℃; the most suitable carbon and nitrogen sources were maltose and tryptone, respectively; and the highest sporulation level was observed under continuous light conditions. This study systematically identifies A. niger as the pathogen of black mold on stored ginger in Sichuan and elucidate its key biological characteristics, providing a scientific basis for the development of green postharvest disease management strategies.

Key words: ginger, Aspergillus niger, pathogen identification, biological characteristics, storage disease

CLC Number:

S435.73

XIAN Yunxi, HU Zijin, HUANG Xiaoqin, YU Yaoying, DENG Yue, SONG Pei, ZHOU Xiquan, LIU Yong, ZHANG Lei, ZHANG Zhongmei, YANG Xiaoxiang. Isolation and Identification of Pathogen Causing Black Mold Disease on Ginger During Storage and Study on Its Biological Characteristics[J]. Chinese Agricultural Science Bulletin, 2026, 42(12): 213-218.

Figures/Tables 8

References 24

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