Biological Characteristics and Pathogenicity of Kiwifruit Black Spot Pathogen

doi:10.11924/j.issn.1000-6850.casb2023-0674

Abstract

Abstract:

In order to confirm the biological and pathogenic characteristics of the pathogenic fungi of black spot on kiwifruit, the biological characteristics of the strain under different culture conditions and their pathogenicity differences to varieties of kiwifruits were studied. It will provide theoretical basis for further research on infection mechanism, epidemic rules and key technologies of disease prevention and control. The results showed that Diaporthe eres, the pathogen of kiwifruit-black spot, could grow at 15-30℃ and pH 5-11. The optimum temperature was 28℃, and it was sensitive to high temperature. The growth rate of the fungus decreased significantly when the temperature was higher than 35℃. The strain could grow in different carbon sources, the most suitable carbon source was glucose. It was easier to use organic nitrogen source, and the most suitable nitrogen source was yeast extract powder. Calcium could promote the growth of the strain, and the growth rate was proportional to the content of calcium. The strain could only reproduce conidia, but could not produce ascospores. The strain had obvious pathogenicity to ‘Cuixiang’, ‘Xuxiang’ and ‘Ruiyu’ kiwifruits, but could not infect ‘Hayward’, ‘Yate’ and ‘Qinmei’ kiwifruits.

Key words: black spot on kiwifruit, Diaporthe eres, biological characteristics, pathogenicity, culture conditions, calcium ion effects

FU Bo, WANG Jiazhe, REN Ping, LI Hao, CHANG Qing, LI Yingmei, ZHANG Feng. Biological Characteristics and Pathogenicity of Kiwifruit Black Spot Pathogen[J]. Chinese Agricultural Science Bulletin, 2024, 40(22): 125-130.

Figures/Tables 8

References 30

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引物名称	引物序列(5’-3’)	退火温度/℃	扩增基因	片段大小/bp
MAT1-1-1FW	GCAAMIGTKTIKACTCACA	50	MAT1-1-1（α1 box结构域）	141
MAT1-1-1RV	GTCTMTGACCARGACCATG	50	MAT1-1-1（α1 box结构域）	141
MAT1-2-1FW	GCCCKCCYAAYCCATTCATC	56	MAT1-2-1（HMG结构域）	229
MAT1-2-1RV	TTGACYTCAGAAGACTTGCGTG	56	MAT1-2-1（HMG结构域）	229

引物名称	引物序列(5’-3’)	退火温度/℃	扩增基因	片段大小/bp
MAT1-1-1FW	GCAAMIGTKTIKACTCACA	50	MAT1-1-1（α1 box结构域）	141
MAT1-1-1RV	GTCTMTGACCARGACCATG	50	MAT1-1-1（α1 box结构域）	141
MAT1-2-1FW	GCCCKCCYAAYCCATTCATC	56	MAT1-2-1（HMG结构域）	229
MAT1-2-1RV	TTGACYTCAGAAGACTTGCGTG	56	MAT1-2-1（HMG结构域）	229