Analysis and Identification of Avirulence Genes of Magnaporthe oryzae from Oryza rufipogon Griff. in Yuanjiang of Yunnan Province

doi:10.11924/j.issn.1000-6850.casb2022-0755

Abstract

Abstract:

In order to clarify the composition of avirulent genes of Magnaporthe oryzae strains isolated from four populations of Oryza rufipogon in Yuanjiang of Yunnan, pathogenicity of 52 single spore strains from O. rufipogon of Yuanjiang were determined on rice blast 24 monogenic lines, and the avirulent genes of these strains were identified by spraying inoculation method at seedling stage. The results showed that among 52 strains of M. oryzae, the proportion of medium, weak and non-pathogenic strains was 34.69%, 65.39% and 1.92% respectively, and the weak-pathogenic strains were dominant. The 52 strains showed the strong or moderate pathogenicity to only five resistant monogenic lines carrying Pi19, Pii, Pi3, Pib and Pish, respectively, and weak or non-pathogenicity to the remaining 19 monogenic lines. On the basis of pathogenicity determination of M. oryzae strains on 24 monogenic lines, the composition of avirulent genes could be divided into 10 types. M. oryzae strains from O. rufipogon in Yuanjiang of Yunan generally showed weak pathogenicity to 24 monogenic lines, but they still appeared pathogenicity differentiations.

Key words: Magnaporthe oryzae, avirulence gene, pathogenicity, Oryza rufipogon, Yuanjiang

DONG Liying, LIU Pei, LIU Shufang, ZHANG Xianwen, YANG Qinzhong. Analysis and Identification of Avirulence Genes of Magnaporthe oryzae from Oryza rufipogon Griff. in Yuanjiang of Yunnan Province[J]. Chinese Agricultural Science Bulletin, 2023, 39(18): 117-122.

Figures/Tables 4

References 22

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单基因系	抗性基因	毒性频率/%	单基因系	抗性基因	毒性频率/%
IRBLA-A	Pia	11.54	IRBLT-K59	Pit	13.46
IRBLI-F5	Pii	71.15	IRBLSH-S	Pish	44.23
IRBL3-CP4	Pi3	71.15	IRBLZ FU	Piz	11.54
IRBLZ5-CA	Pi5	0.00	IRBLZT-T	Piz-t	9.62
IRBLKS-S	Pik-s	0.00	IRBL9-W	Pi9	3.85
KRBLK KA	Pik	0.00	IRBLZ5-CA	Piz5	15.38
KRBLKP-K60	Pik-p	0.00	IRBLTA-K1	Pita	0.00
KRBLKH-K3	Pik-h	0.00	IRBLTA2-PI	Pita-2	3.85
IRBLKM-TS	Pik-m	0.00	IRBL12-M	Pi12	13.46
IRBL1-CL	Pi1	0.00	IRBL19-A	Pi19	86.54
IRBL7-M	Pi7	0.00	IRBL20-IR24	Pi20	3.85
IRBLB-B	Pib	28.85	IRBL11-ZH	Pi11	9.62

单基因系	抗性基因	毒性频率/%	单基因系	抗性基因	毒性频率/%
IRBLA-A	Pia	11.54	IRBLT-K59	Pit	13.46
IRBLI-F5	Pii	71.15	IRBLSH-S	Pish	44.23
IRBL3-CP4	Pi3	71.15	IRBLZ FU	Piz	11.54
IRBLZ5-CA	Pi5	0.00	IRBLZT-T	Piz-t	9.62
IRBLKS-S	Pik-s	0.00	IRBL9-W	Pi9	3.85
KRBLK KA	Pik	0.00	IRBLZ5-CA	Piz5	15.38
KRBLKP-K60	Pik-p	0.00	IRBLTA-K1	Pita	0.00
KRBLKH-K3	Pik-h	0.00	IRBLTA2-PI	Pita-2	3.85
IRBLKM-TS	Pik-m	0.00	IRBL12-M	Pi12	13.46
IRBL1-CL	Pi1	0.00	IRBL19-A	Pi19	86.54
IRBL7-M	Pi7	0.00	IRBL20-IR24	Pi20	3.85
IRBLB-B	Pib	28.85	IRBL11-ZH	Pi11	9.62