Abstract: Having cultured the stem sections of Kalanchoe blossfeldiana in vitro for7-8days, the axillary buds of explants can germinate. The result of culturing for 30 days showed that the most suitable medium to induce buds was MS+6-BA1.0+NAA0.5 mg?l-1, the most suitable one to make the bud stretch was 1/4MS, and to form available knots was MS. During the continuing culture, the germinating rate of stem sections with no leaves was higher than that of the sections with leaves on all the above media. Adventitious buds began to form at the base of petiole after having been cultured for 9 days on the media No.5,6,7. On the other media, however, they only began to form after being cultured for 14days. Simultaneously, the most suitable medium to induce the leaves to regenerate adventitious buds was 1/2MS+6-BA1.0 mg?l-1, and to induce the roots to grow was 1/4MS.