Abstract: This studies utilized foreign germplasms resources (Sprite87,Hobbit) as the key parents, and utilized local soybean variety Hefeng 42 as the improvement parents. Based on the crossbreeding and genetic improvement, and combined with radiation breeding techniques, through continuous directional selecting and using quality analysis and soybean disease-resistant, three high-oil new germplasms were bred, they are Hefu04-40, Hefu04-46 and Hefu04-52. their oil contents are 23.95%~24.20%, the yields are 2559~2779.5kg/hm2,and the yield increased 11.6%~21.2% compared to check. This paper reported the results of creating soybean high-oil germplasms.