Abstract: Effects of temperature, moisture, pH and culture media on the growth of Aspergillus tamarii Kita, Eurotium repens de Bary and Eurotium chevalieri Mangin, which cause mildew of store tobacco, were studied. For the growth of A. tamarii, E. repens and E. chevalieri, the range of temperature were 16~43℃, 13~31℃ and 16~37℃, and the optimum were 22~34℃, 22~28℃ and 22~31℃ respectively. Moisture was the most important factor for the growth of these fungi. E. repens and E. chevalieri started to grow slowly when the relative humidity reached 75%, while A. tamarii started to grow when the relative humidity reached 82%, and the higher the moisture was, the faster the growth of these fungi became. All of them have wide adaptability to pH, they could grow even at pH 2.5. A. tamarii grew fast on artificial media, while E. repens and E. chevalieri grew very slowly, but all of them grew fast on natural tobacco leaves media. The lethal temperature for A. tamarii was 60℃ remaining 20 minutes or 65℃ remaining 10 minutes， while for E. repens was 65℃ remaining 30 minutes or 70℃ remaining 5 minutes and for E. chevalieri was 70℃ remaining 15 minutes.