Abstract: In order to breed quality Burley tobacco varieties, which were appropriate for planting at different region, the field comparison test of 5 plant lines and CK were conducted and their agricultural and economic character, disease resistance were analysized. The agricultural character results showed that the plant height were higher than that of CK besides 99P-2-1-1 and 99K-5-2-1 was the highest in the 5 plant lines. The average leaf number was 22.6,which was close to CK. The growing stage of all plant lines were 97d, which were middle maturing plant line. The resistance date indicated that tobacco black shank disease, tobacco brown spot were found on all plant lines. The disease incidence of 99I-7-5-1 tobacco black shank disease was the highest in the 5 plant lines, and that of 99K-5-2-2 was the lowest. The disease incidence of 99F-1-1-2 tobacco brown spot was the highest in the 5 plant line, and that of 99P-2-1-1 was the lower. The disease incidence of the tobacco mosaic virus all were 0.65% besides 99I-7-5-1, TN86(CK) being 0. The yield of 99K-5-2-2、99K-5-2-1 overtook that of CK, and the yield of 99K-5-2-2 was the highest, and that of 99P-2-1-1 was second. The output exceeded CK besides 99I-7-5-1, and that of 99K-5-2-2 the highest, and that of 99P-2-1-1 was second. The first-class tobacco proportion of 99K-5-2-2 was the highest. The genetic character of 99K-5-2-2, 99K-5-2-1, 99F-1-1-2 was stable, and the plant type was rational. The comprehensive resistances were the highest. The yield and production value and high and middle class leaf ratio were than that of CK or close to CK. The usefullness of cured leaf was high. Above all, the comprehensive performance 99K-5-2-2, 99K-5-2-1 of was the best. 99F-1-1-2 was Second.