Abstract: Tissue culture root seedlings of a new Asparagus variety-“GIJNLIM” were taken experimental materials. Studying the effects of transplant matrix , the sterilization of matrix, take root type and transplant stages on transplant survival percent of asparagus seedlings.The results showed that in the south of china, tissue culture root seedlings cultivated in the two-step rooting method were used; coco bran as to transplant matrix, transplant matrix was sterilized by GENBINGZHI 200g/m3 in Spring and Winter, the transplant survival percent attains above 95.20%~99.25%.