Identification on the Main Cultivated Varieties of Pyrus Using SSR DNA Marker

Abstract

Abstract: It is very important for the farmers and breeders to build a fast and dependable method of cultivars identification. The publicly available collection of expressed sequence tags in GeneBank from fruit trees can be used in the analysis of developing simple sequences repeat (SSR) markers. In the present paper, a dichotomous key to 19 cultivars of pear was provided. Totally 19 individuals were selected and analyzed by amplifications using 6 pairs of SSR primers screened. 19 cultivars could be distinguished from each other with this fingerprinting map. The way of expressed sequence tags in GeneBank was a simple and fast technique to SSR markers, which could highly deduce the cost of progress of SSR Primers and the time of reaction system establishment.

CLC Number:

S661.2

Han Hongwei, Yang Minsheng, Xu Xingxing, Liang Haiyong. Identification on the Main Cultivated Varieties of Pyrus Using SSR DNA Marker[J]. Chinese Agricultural Science Bulletin, 2006, 22(12): 383-383.

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Identification on the Main Cultivated Varieties of Pyrus Using SSR DNA Marker

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