Abstract: The present work studied the effects of spaceflight treatment on Gerbera jamesonii seed germination, in vitro culture and micropropagation. Our results indicated that the germination of the treated seeds was two or three days faster than that of no-treated seeds, but the germination ratio either enhanced or not. The influences on shoot tip cultures were different between two spaceflight treatments, one inhibited the micropropagation and the other acted as an opposed role. The 4~5 generation of the subculture had no different phenotype from the wild-type culture. Importantly, almost all tissue-culture-plants of treated seeds were characterized by etiolation, superdwarf, leaf aberrance, and some shoot tips and calli from the leaves of these cultures were prone to produce a plenty of roots with many branches.