Abstract: Effect of the plant hormones and active carbon on rooting of in vitro shoots of Saussurea involucrata Kar. et Kir. was studied. Four rooting indexes, including root length, root thick, rooting number and rooting induction rate, were measured. It was concluded that adding the auxins, such as IAA, IBA and NAA, could enhance the induction of the roots, and 0.2mg/L IAA could produce the best result, the length of root is longest (5.5mm), the root thick is the thinnest (0.96mm), rooting number is much more than others, the rooting induction rate is 100%. Adding active carbon could improve the quality of the rooting. The optimal concentration was 1mg/mL, the length of root is 25.51mm, the root thick is 0.6mm, and rooting number is 13.