use Qian-Tang 4 for supplying experiment materals Choose the axillary buds on the young shoots of sugarcane for being tissue culture of inoculation from outward plant body to be parted from body utilize 75% alcohol steep 8~10S and 0.1% Hgcl2 antisepsis 15 min multilication antisepsis Clean again and again, then inoculate In axillary buds inducing culture utilize 1/2 MS+0.25mg/L(6-BA) (tiquid state); In thicket shoot indncing to increase culture, utilize MS+2.5mg/L(L-B)+0.2mg/L(KT) (solid state) Continue to change a generation once every 15-20d Breeding multiple is more than 30 times During growing root time utilize 1/2 MS+30mg/L (NAA)+0.1mg/L(IBA) (solid state) Append 2.5mg/L MET in order to make seedings strong After refining seedings transplant them on the seed bed of sand-soil-sand for hardening, then harden individual plant to about 20cm high Finally let it be planted in the fields so groke a set of quick muliplication seedings skiu of Qian-Tang 4 axillary buds which are parted from body And introduce operate essentials particularly Pose relevant links that we should pay attention to five problems of them and try to find good ways to solve
