Rhododendron fortunei is a wild ornamental plant with highly appreciative and useful value. In vitro micropropagation technology of Rhododendron fortunei was aimed to be established using shoots as explants and WPM as fundamental culture medium. The results indicated that axillary shoots could be induced by the medium supplemented with a concentration of 1~5 mg/L zeatin, and it needed 6~8 weeks for one regeneration. Different concentration of zeatin had a significant influence on regenerated shoots. The best hormone concentration for shoot regeneration was at 0.5~1 mg/L from quantitative and qualitative views. The pH value which had obvious effect on shoot growth was appropriate at 4.8~5.2, although showing no significant difference in regenerated shoots. In vivo rooting percentage of 85% was obtained when unrooted shoots were directly transplanted into soilless mixtures.
