This paper uses the comprehensive comparative advantage indexes formed from yield and scale basis to conduct calculation and analysis on the comparative advantages of 6 major grain crops at various cities. The results indicate that there exist distinguish regional differences in the grain crops production in Anhui province. The government should completely exploit the comparative advantage and gain from specialization in agricultural production by guiding the producers to adjust their production structure according to the law of comparative advantage. Meanwhile, the paper gives scientific and direct suggestion to grain production re-arrangement among regions and varieties.