Abstract: Based on the climate and soil conditions of Henan province，the physiologic functions of Zn and Mn and the existing problem of alfalfa（Medicago sativa L.）production，this paper studied the responses to the coordinate use of two trace element fertilizers（Zn and Mn）in alfalfa grassland，and analyzed the applied range and concrete measures. The results showed that effective applied concentration ranged from 400mg/kg to 500 mg/kg with the optimum concentration being 430mg/kg. Coordinate use of Zn（430mg/kg）and Mn（380mg/kg）could increase the forage yield by 10.7% and the seed yield by 13.7%, respectively, showed a better productive effect.