Abstract: We studied the peony disease which planted in the Hunan Changsha, and found that the main diseases of peony in Hunan were peony grey mildew, Peony brown spot, Peony red spot disease, Peony branch-rot disease and peony root-rot disease. We found that there was a peak between June and August with rainfall amount increase through system investigation. Through medicament screening in disk, we found Mancozeb and Prochloraz can inhibit these pathogen growths effectively.