Abstract: To investigate the effect of levamisole on antibody tiger of ND vaccine, the testing chicken was divided into four groups named group A、B、C and D, 5 chick/group. A、B、C group were treated with Levamisole and Newcastle disease vaccine. A group was high dose including 0.6mg Levamisole, B middle dose 0.3mg, C low dose 0.15mg, and D control. The blood samples were collected at 7d,14d, 21d, 28d after inoculation, and analyzed with hem-agglutination inhibition (HI). The results showed that the antibody titer of the four group was of no difference before immune. The antibody titer among high, middle, and low dose group was not different statistically at the 7d, 14d and 21d after immune. The antibody titer of the middle dose group was higher at 28d after immune. Statistics and analyses proved that the control group was different from other 3 groups, which implied that the groups treated with Levamisole were greatly significant. However it is not relative to dose of Levamisole. B middle dose group displayed the best performance among A、B、C group, and the antibody value was high (7.2log2), but the difference was not remarkable compared with A、C group. This indicated that addiction of Levamisole in ND vaccine could promote antibody titer, and titer tendency was upward along with time.