Abstract: The hapten of deltamethrin was synthesized by chemistry method, the results of the 1H-NMR indicated that the hapten was synthesized successfully. Hapten was conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) by diazotization method, two artificial antigens were used as antigen and coating antigen. Antigens were confirmed by ultraviolet spectrum. The coupling ratio were 11:1 and 8:1.Two rabbits were immunized, the antiserum was examined by indirect ELISA and indirect competitive ELISA experiments, the titer of the two rabbits was 100000 and 80000 separately, the inhibition ratio was more than 80% when the concentration of the deltamethrin was 50μg/ml. The results showed that the artificial antigen was synthesized successfully.