Abstract: Abstract： The content of total flavonoids in fruit of Castanea henryi was distilled with ethanol and determined at 509nm by ultraviolet spectrophotometry with rutin as a standard contro1 by orthogonal design. The results show that the average recovery of total flavonoids was 94.3％ with the relative standard deviation 0.61％( =6). The method is simple，rapid and accurate. It can be used for determining the content of total flavonoids in fruit of Castanea henryi.