Abstract: one Phosphate solubilizing fungi named F2 was cultured in shake flasks containing the media with Ca3 (PO4)2 or lecithin as the only P source for 132 hours, with the temperature is 25℃ and the shaking speed is 180r/min,in different time, determine the pH、the concentration of dissoluble phosphorous、the weight of mycelium and the absorbed concentration of phosphorous by F2.The results showed that with the cultured of fungi F2, the pH value of the liquid fermentation has dropped, the ability of dissolving phosphate has increased, the weight of mycelium has increased. The results showed that the concentration of dissoluble phosphorous was negatively correlated with pH; the pH was negatively correlated with the weight of mycelium;F2 was well in dissoluble abiophosphorous; after shaking 108h,F2 was mature ,then it’s good for dissoluble phosphorous.