Abstract: Abstract: In order to study on the changing condition of some contents of the medium and trace elements in tobacco leaves in Guiyang area in recent years, detected the medium and trace elements contents of different positions of tobacco leaves at Heping and Qiaoshi townships of Guiyang area in 2005 and 2007 with the methods of ICP and compared with that of Zimbabwe. The results showed that, compared with that in 2005, the contents of B, Fe and Cu were decreased evidently and were at a lower level in 2007, and at the same time the content of Mn was slightly lower but with a moderate content. Compared with the tobacco leaves of Zimbabwe, the tobacco leaves of Guiyang area were poor in B, Fe and Cu. The study suggests to increase the application amount of the medium and trace elements in Guiyang area.