Abstract: The effect of sucrose concentrations, boric acid, temperature, pH value and different storage conditions on pollen germination of sweet persimmon cv. Zenjimaru in vitro were studied. The results as follows: 25℃, 10% sucrose, 50mg/L boric acid and pH 6 were the optimal conditions for pollen germination, in which the rate of pollen germination was 69.53%. The suitable temperature for keeping pollen viability was -20℃ freeze dehydration, followed by -20℃ freeze, 4℃ low-temperature dehydration, 4℃ low-temperature, normal- temperature dehydration and normal-temperature. So reference basis for pollen-storage and artificial pollination of sweet persimmon in the production was offered.