Abstract: （OBJECTIVE）To improve the yield of triterpene in callus of birch, (METHOD）oleanolic acid as standard, 5% vanillin-acetic acid and perchloric acid as the developer, the determination method of triterpene was evaluated, the results showed that this method had a higher repeatability, and quite stable during 5~50min in color reaction; The triterpene in callus with ultrasonic was extracted under 20℃，40℃，60℃，80℃, the content of triterpene was 40℃（1.67mg/g）＞ 60℃（1.28 mg/g）＞ 80℃（0.47 mg/g）＞ 20℃（0.08 mg/g）. The triterpene in callus was extracted with benzene, acetone, ether, ethanol95%, methanol, chloroform, the content of triterpene was benzene（2.58 mg/g）＞ ether（2.04 mg/g）＞ ethanol（1.67 mg/g）＞ chloroform（1.21 mg/g）＞ methanol（0.89 mg/g）＞ acetone（0.50 mg/g）. （CONCLUSION） It is proposed that the best extration temperature and reagent was 40℃ and ethanol95%,respectively.