Abstract: The colour of Geranium pretense’s flower is fresh,also its inflorescence and flowering time are longer.Owing its higher stress tolerance, Geranium pretense is excellent Garden plant.But it is growing in wildness such as frorest now . Introduction and domestication of it frequently destroy wild resource of Geranium pretense. Isolated culture can produce evenly sterility seedlings in short time,it is base for introduction and domestication or application in gardens.This paper established Geranium pretense’s culture in vitro used seedings of Geranium pretense. The effect of different accretion on browing of Geranium pretense was studied and the optimal medium composition for rooting and proliferation of Geranium pretense were selected by comparison the effect of different medium culture on growing of Geranium pretense. These results showed that, the seeds of Geranium pratense were sterilizated by 20% NaClO for 1.5 min was efficiency;Browning was alleviated by active carbon and longer timing culture in vitro;The optimal medium composition for rooting was 1/2MS+AC 2g/L and 1/2MS+ 6-BA0.5+NAA0.05 was suit for proliferation of Geranium pretense.These results will providing theoretica base for rapid propagation .