Abstract:

The corm of Zabtadeschia hybrida was used as explants, establish Zabtadeschia hybrida in vitro rapid propagation system for adventitious buds. The results showed that adopted “secondary sterilize” can reduce the rate of pollution in initial culture, and establish Zabtadeschia hybrida in vitro rapid propagation system for adventitious buds was feasible, the optimum induction culture medium of adventitious buds was MS + 1 mg/L 6-BA + 0.1 mg/L NAA, and the multiplication adventitious bud of culture medium was MS + 2 mg/L 6-BA +1 mg/L NAA, and the optimum rooting medium was 1/2MS + 0.1 mg/L 6-BA + 0.5 mg/L NAA+0.5 mg/L AC. After 3 days of acclimation and sun-shading treatment it can obtain ratio of survivals more than 90%.