Abstract: Abstract: Wheat Sharp eyespot (Rhizoctonia cerealis vander hoeven) has already been the main disease of wheat in the Yellow-Huai and Yangtze river valley region, it is frequently and severely occurred recently. Screening of resistant materials is the prerequisite of wheat breeding for controlling the disease. In this paper we implemented two inoculation methods, the toothpick embedding and grain embedding methods, respectively,, and found the grain embedding method had better effect than that of toothpick. 439 wheat from Yellow-Huai and Yangtze river valley and CIMMITY were then inoculated by using grain embedding method, results showed that materials were different in resistant level obviously. The strategic of breeding for controlling sharp eyespot was also discussed.