Abstract: Abstract: OBJECTIVE The fermentation broth of marine sponge-associated actinobacteria Streptomyces sp. A01059 was studied for the valuable active compounds which against rice blast; METHOD The fermentation broth was extracted with ethyl acetate, then activity-guided method was used to analysis the extraction and the active compounds were obtained by preparative liquid chromatography; RESULTS Two active compounds were obtained and the molecular weight were 508 and 522, respectively; CONCLUSION The metabolites of Streptomyces sp. A01059 were studied in this paper for the first time systematically, and a new method was provided to control rice blast.