Abstract:

Abstract：The young leaves culture and the rapid propagation of Kalanchoe blossfeldiana were carried out and media for various culture stages were selected. The experimental results indicated that explant sterilized in 0.1％ aqueous mercuric chloride for 4 min has best effect,with the contamination rate 12.5% , and the death rate 2.5%. The best inducement culture medium is MS+6-BA1.0 mg／L+NAA0.1～0.3 mg／L. The suitable culture medium for bud differentiatio and proliferatio is 1/2MS + 6-BA1.0 mg／L + NAA0.1 mg／L. The plantlets are transplanted into nutrient matrix with 99％ survival ratio.