Abstract:

Abstract:【OBJECTIVE】Study on the effect of Polyethyleneglycol 6000(PEG6000) osmotic treatment on seed germination and seedling growth of Echinochloa crusgalli. Analysis the interaction among the seed germination,seedling growth and water stress. Accelerating the initiate effect of the germination and the viability of seeds, provding the scientific proof to the Production Planning for Echinochloa crusgalli.【METHOD】Echinochloa crusgalli seeds were pretreated with various concentration of PEG6000(15%,20%,25%,30%) for different durations (24h、60h、100h ) to investigate their effects on germination(G), rapidity of germination(RG), relative electric conductivity (REC) and proline content (Pro C).【RESULTS】the adequate concentration of PEG6000 and the appropriate pretreating time could accelerate the germination of Echinochloa crusgalli seeds in varying degrees. The effects were characterized by the improvement of G, increased RG, descended REC. while the Echinochloa crusgalli seeds were pretreated by 25% PEG6000 for 24h, the Pro content could achieve 633.31 ug/mg, which is 3.56 times of that of CK. This indicates significant difference between them (P＜0.01).【CONCLUSION】PEG6000 osmotic treatment could improve G,RG and integrity of cell membrane system. The optimal conditions to initiate the germination of Echinochloa crusgalli seeds were as follows: 25% PEG osmotic treatment and 24h duration.