Abstract:

Abstract: In this study, In view of the endogenous reference gene ANS and the exogenous genes such as F3'5'H, CHS promoter, D8ter, the gene and construct–specific qualitative PCR detection methods were established for transgenic carnations——Moonshade、Moonlite which were developed by Japanase Suntory company and Australian Florigene company. In addition, PCR primers were designed respectively in the exogenous MAC gene promoter and the DFR gene, construct–specific qualitative PCR detection was carried out. The detection sensitivity reached 0.50%. The establishment of this method provides preliminary data for the transgenic carnation import detection, domestic supervision and environmental safety assessment..