Abstract: Abstract: Three different methods were used to extract genomic DNA from sugarcane seed-stem , and the methods were CTAB, SDS-Ⅰand SDS-Ⅱ. Leaves were taken as the control. The quality of the extracted DNA was detected. The results showed that the DNA yield of SDS-Ⅰwas the highest. Overall, although the yield of DNA from seed-stem was lower than leaves, but the concentration and purity of DNA from seed-stem were sufficient to meet the requirement of the downstream molecule experiments, and the operation was more convenient than taking leaves as the material.