Abstract:

Effects of plant hormones to the tissue culture of Quercus acutissima were studied by means of seedling stems as explants and MS as basic medium. The results showed that the best medium for the bud induction was MS +6-BA1.0 mg?L-1. The bud was as long as 1 to 2 cm with 3 to 4 leaves after three weeks, and the germination rate was 83.3%. The best medium for the bud proliferation was MS +6-BA1.0 mg?L-1 + NAA0.02 mg?L-1, and the proliferation rate was 4.6. The best medium for rooting was 1/2MS + NAA0.5 mg?L-1, and the rooting rate was about 87% after four weeks.