Abstract:

Resistance evaluation method which convenient to compare results between years and researchers is the foundation of screening stable resistant germplasm. By artificial inoculation in plastic tunnel, resistance evaluation method of tobacco germplasm to potato virus Y was compared. The compared results of Disease index(DI), Relative disease index(RDI), Relative resistance index(RI), and Area under the disease progress curve(AUDPC) show that RDI could directly reflect germplasm resistance, and convenient to compare results between years and researchers. The resistance results of germplasm evaluted by high pressure spray mechanical inoculation and aphid feeding inoculation are consistent each other. By evaluated through high pressure spray mechanical inoculation in plastic tunnel, NC55 and NC102 exhibit high resistance, Virginia SCR(VSCR) and TI245 exhibit resistance, CV91 and Oxford 207 exhibit medium resistance, Taiyan 6 exhibit medium sensitive, NC297and Yunyan 97 exhibit sensitive, Coker176 exhibit high sensitive. The entities F1 of resistant parent NC55, VSCR and sensitive parent exhibit sensitive or high sensitive, indicating that the resistance of NC55 and VSCR to PVY are recessive heredity.