Abstract:

The pollen germination rate (PGR) is one of the most important factors in evaluating the pollen vitality. Practically, low-cost, high-speed and feasible culture methods for determination of PGR are lacking. In the present study, we tried to screen the ingredients of a liquid culture medium for rice pollen germination test based on some previous studies. The results indicated that, with a two-line hybrid rice cultivar Liangyou 818, pollen germination rate in the culture medium with a ingredient of 155g/L sucrose +20mg/L H3BO3+40mg/L CaCl2+5 mg/L VB1 showed significantly greater than the other ingredient-based media, with a highest PGR 63.78%. In addition, this culture method was characterized by low-cost, stability in PGR, and evenly distribution of pollens in the surface of the culture medium.