Abstract:

nested-PCR was used to detect citrus Huanglongbing (HLB) in Clausena lansium and 11 citrus varieties. A specific 400 bp band was amplified from plant materials, respectively. The result demonstrated that Clausena lansium and 11 citrus varieties from Guangxi could be infected by HLB. Simultaneously, 16S rDNA of HLB pathogen was amplified and sequenced. Homology analysis and cluster analysis indicated that HLB from Guangxi belonged to ‘Candidatus Liberibacter asiaticus’, and was more closed to Xiamen lines from China and two Japanese lines.