Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2010, Vol. 26 ›› Issue (22): 27-33.

Special Issue: 生物技术

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Molecular Cloning and Expression Analysis of Cu/Zn Superoxide Dismutase Gene (MrSOD1) cDNA from Myrica rubra

  

  • Received:2010-09-26 Revised:2010-10-14 Online:2010-11-20 Published:2010-11-20

Abstract:

A full-length cDNA sequences of Cu/Zn-SOD was cloned from Myrica rubra leaves by using the RT-PCR technology, and the expression levels of this gene in different tissues were found through Semi-Quantitative RT-PCR method. The full-length is 461 bp encoding a 152 amino acids Cu/Zn-SOD ortholog. The sequences contained the Cu/Zn-SOD family signature sequence and had the homology more than 77% comparing with other Cu/Zn-SOD from GenBank. This gene was named as MrSOD1 (GenBank accession No. GQ404510). The expression levels of MrSOD1 in different tissues were fruit>leaf>flower >shoot. The study provided a way for deep research on the expression and regulation of MrSOD1, and the molecular mechanism of anti-oxidative effect in Myrica rubra.